Hotspot

trnL-CAA-ndhB

IRb · 99335-99968 · 634 bp

Back to Triumfetta

Pi 0.00315

MarkerSeek score 55.00

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: IRb
length_bp: 634
variable_sites: 6
pi: 0.00315
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	GTCTCGCGTGTCTACCATTT	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	688-695 bp	1.0	81.2
trnL-CAA-ndhB_p2	CTCGCGTGTCTACCATTTCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	686-693 bp	1.0	81.2
trnL-CAA-ndhB_p3	TACCATTTCACCACCAAGGC	TGCAATTGCTCAGGATACCC	50.0	50.0	58.1	57.9	676-683 bp	1.0	80.6
trnL-CAA-ndhB_p4	GCGTGTCTACCATTTCACCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.2	57.9	683-690 bp	1.0	79.7
trnL-CAA-ndhB_p5	TCTCGCGTGTCTACCATTTC	TGCAATTGCTCAGGATACCC	50.0	50.0	57.7	57.9	687-694 bp	1.0	79.1