Hotspot

ndhC-trnC-ACA

LSC · 53801-54864 · 1064 bp

Back to Triumfetta

Pi 0.00636

MarkerSeek score 57.40

Primer available yes

Label ndhC-trnC-ACA

Feature Metadata

label_name: ndhC-trnC-ACA
feature_type: igs
region: LSC
length_bp: 1064
variable_sites: 17
pi: 0.00636
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnC-ACA_p1	ACCCTTTCTAATCGGGGCTA	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.8	57.9	1142-1198 bp	1.0	83.1
ndhC-trnC-ACA_p2	CGGGGCTAAAACTCCAGAAA	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.8	57.9	1130-1186 bp	1.0	83.0
ndhC-trnC-ACA_p3	ACCCTTTCTAATCGGGGCTA	CGGTTCGAATCCGTATAGCC	50.0	55.0	57.8	58.3	1143-1199 bp	1.0	81.2
ndhC-trnC-ACA_p4	ATCGGGGCTAAAACTCCAGA	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.4	57.9	1132-1188 bp	1.0	81.2
ndhC-trnC-ACA_p5	AATCGGGGCTAAAACTCCAG	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.6	57.9	1133-1189 bp	1.0	81.2