Hotspot

atpH-atpI

LSC · 14257-15454 · 1198 bp

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Pi 0.00469

MarkerSeek score 57.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1198
variable_sites: 15
pi: 0.00469
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	1255-1257 bp	1.0	79.2
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	CGAATCCATGGAGGGTCATC	50.0	55.0	57.9	57.8	1311-1313 bp	1.0	78.6
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.2	57.8	1256-1258 bp	1.0	78.1
atpH-atpI_p4	GCAGTACCTTGACCAACTCC	CGAATCCATGGAGGGTCATC	55.0	55.0	58.2	57.8	1315-1317 bp	1.0	78.0
atpH-atpI_p5	GGCAGTACCTTGACCAACTC	CGAATCCATGGAGGGTCATC	55.0	55.0	58.2	57.8	1316-1318 bp	1.0	78.0