Hotspot

atpF-atpH

LSC · 13396-14010 · 615 bp

Back to Triumfetta

Pi 0.0116

MarkerSeek score 67.80

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 615
variable_sites: 24
pi: 0.0116
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCAGTGACCCAAAGAAACGA	TGGTTGTAGCATTGGCACTT	50.0	45.0	58.0	58.0	689-715 bp	1.0	85.4
atpF-atpH_p2	CCAGTGACCCAAAGAAACGA	TTGGTTGTAGCATTGGCACT	50.0	45.0	58.0	58.0	690-716 bp	1.0	85.4
atpF-atpH_p3	CCAGTGACCCAAAGAAACGA	GTTGGTTGTAGCATTGGCAC	50.0	50.0	58.0	57.9	691-717 bp	1.0	85.0
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	TGGTTGTAGCATTGGCACTT	50.0	45.0	57.9	58.0	716-742 bp	1.0	84.6
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	TTGGTTGTAGCATTGGCACT	50.0	45.0	57.9	58.0	717-743 bp	1.0	84.6