Hotspot

rpl23

LSC · 55636-56919 · 1284 bp

Back to Triticum

Pi 0.0024

MarkerSeek score 46.90

Primer available yes

Label rpl23

Feature Metadata

label_name: rpl23
feature_type: gene
region: LSC
length_bp: 1284
variable_sites: 12
pi: 0.0024
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23_p1	ACCATTTATGCGCTGGAGAG	GACTCACGATTACGCCTGTT	50.0	50.0	58.0	58.0	1495-1506 bp	1.0	80.6
rpl23_p2	ACCATTTATGCGCTGGAGAG	GTTGACTCACGATTACGCCT	50.0	50.0	58.0	58.0	1498-1509 bp	1.0	80.6
rpl23_p3	ACCATTTATGCGCTGGAGAG	TTGACTCACGATTACGCCTG	50.0	50.0	58.0	58.0	1497-1508 bp	1.0	80.6
rpl23_p4	CACAACCATTTATGCGCTGG	GACTCACGATTACGCCTGTT	50.0	50.0	58.1	58.0	1499-1510 bp	1.0	80.2
rpl23_p5	CACAACCATTTATGCGCTGG	GTTGACTCACGATTACGCCT	50.0	50.0	58.1	58.0	1502-1513 bp	1.0	80.2