Hotspot

rpl32-trnL

SSC · 104450-105138 · 689 bp

Back to Triticum

Pi 0.00598

MarkerSeek score 47.80

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 689
variable_sites: 19
pi: 0.00598
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	GAGCGGTAATGAGCATCCAA	TTCGAACCGAGATGCTTGAG	50.0	50.0	58.0	58.0	845-858 bp	1.0	81.5
rpl32-trnL_p2	TTTTCGAGCGGTAATGAGCA	TTCGAACCGAGATGCTTGAG	45.0	50.0	57.9	58.0	850-863 bp	1.0	81.2
rpl32-trnL_p3	GAGCGGTAATGAGCATCCAA	GGATTCGAACCGAGATGCTT	50.0	50.0	58.0	58.1	848-861 bp	1.0	81.0
rpl32-trnL_p4	AAGGGTTTTTCTGGGCAACA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	820-833 bp	1.0	80.9
rpl32-trnL_p5	AGGGTTTTTCTGGGCAACAA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	819-832 bp	1.0	80.9