Hotspot

petA-psbJ

LSC · 60584-61403 · 820 bp

Back to Triticum

Pi 0.00341

MarkerSeek score 51.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 820
variable_sites: 23
pi: 0.00341
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCATTACGTGTTCAAGGCCT	GGTACTGTAGCCGGTATTGC	50.0	55.0	58.2	58.1	1016-1024 bp	1.0	81.4
petA-psbJ_p2	TCCATTACGTGTTCAAGGCC	GGTACTGTAGCCGGTATTGC	50.0	55.0	58.2	58.1	1017-1025 bp	1.0	81.3
petA-psbJ_p3	CCATTACGTGTTCAAGGCCT	GTAGCCGGTATTGCTGTGAT	50.0	50.0	58.2	57.8	1010-1018 bp	1.0	80.6
petA-psbJ_p4	TCCATTACGTGTTCAAGGCC	GTAGCCGGTATTGCTGTGAT	50.0	50.0	58.2	57.8	1011-1019 bp	1.0	80.5
petA-psbJ_p5	ACGTGTTCAAGGCCTTTTGA	GGTACTGTAGCCGGTATTGC	45.0	55.0	58.2	58.1	1016 bp	0.043478	38.7