Hotspot

psbI-trnS

LSC · 7795-7907 · 113 bp

Back to Triticum

Pi 0.00539

MarkerSeek score 47.30

Primer available yes

Label psbI-trnS

Feature Metadata

label_name: psbI-trnS
feature_type: igs
region: LSC
length_bp: 113
variable_sites: 3
pi: 0.00539
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS_p1	TCTAATGACCCAGGACGGAA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	57.8	58.0	215-220 bp	1.0	80.7
psbI-trnS_p2	TCTAATGACCCAGGACGGAA	GCTTTGGAACGTGGAGAGAT	50.0	50.0	57.8	57.9	247-252 bp	1.0	79.8
psbI-trnS_p3	TCTAATGACCCAGGACGGAA	ATCACGGAAACCTTTCGCTT	50.0	45.0	57.8	58.1	263-268 bp	1.0	79.6
psbI-trnS_p4	ATCTAATGACCCAGGACGGA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	57.5	58.0	216-221 bp	1.0	78.8
psbI-trnS_p5	TCTAATGACCCAGGACGGAA	CACGGAAACCTTTCGCTTTG	50.0	50.0	57.8	58.2	261-266 bp	1.0	78.5