Hotspot

rpl32-tRNA-Leu(UAG)

SSC · 124983-125972 · 990 bp

Back to Trithuria

Pi 0.182
MarkerSeek score 74.60
Primer available yes
Label rpl32-tRNA-Leu(UAG)

Feature Metadata

label_name
rpl32-tRNA-Leu(UAG)
feature_type
igs
region
SSC
length_bp
990
variable_sites
110
pi
0.182
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.61
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-tRNA-Leu(UAG)_p1 AGGTGCCATGTTTTGTCTGA TCTTCCCGCAATTCCAACAA 45.0 45.0 57.6 58.0 1502 bp 0.5 57.0
rpl32-tRNA-Leu(UAG)_p2 AGGTGCCATGTTTTGTCTGA GCTATCTTCCCGCAATTCCA 45.0 50.0 57.6 58.0 1506 bp 0.5 56.8
rpl32-tRNA-Leu(UAG)_p3 CGCGTGCCTATCTCTATGTT TCTTCCCGCAATTCCAACAA 50.0 45.0 57.6 58.0 1421 bp 0.5 56.5
rpl32-tRNA-Leu(UAG)_p4 CGCGTGCCTATCTCTATGTT GCTATCTTCCCGCAATTCCA 50.0 50.0 57.6 58.0 1425 bp 0.5 56.3
rpl32-tRNA-Leu(UAG)_p5 AGGTGCCATGTTTTGTCTGA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.6 57.9 1433 bp 0.5 56.1