Hotspot

ndhF-rpl32

SSC · 123958-124829 · 872 bp

Back to Trithuria

Pi 0.153

MarkerSeek score 83.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 872
variable_sites: 125
pi: 0.153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACGAAAGGGATAATCCACGC	TTCCGATTCCTTCAGCAACC	50.0	50.0	58.1	58.2	1088 bp	0.5	58.2
ndhF-rpl32_p2	CGAAAGGGATAATCCACGCA	TTCCGATTCCTTCAGCAACC	50.0	50.0	58.1	58.2	1087 bp	0.5	58.2
ndhF-rpl32_p3	TTGCGCCGATTTAGCAATTG	TTCCGATTCCTTCAGCAACC	45.0	50.0	58.1	58.2	1776 bp	0.5	57.8
ndhF-rpl32_p4	CATTCCCACGAGTTCCCAAA	TTCCGATTCCTTCAGCAACC	50.0	50.0	58.1	58.2	1515 bp	0.5	57.8
ndhF-rpl32_p5	ACGAAAGGGATAATCCACGC	CGATTCCTTCAGCAACCCAT	50.0	50.0	58.1	58.2	1085 bp	0.5	57.6