Hotspot

ndhC-tRNA-Val(UAC)

LSC · 50567-51670 · 1104 bp

Back to Trithuria

Pi 0.141

MarkerSeek score 84.60

Primer available yes

Label ndhC-tRNA-Val(UAC)

Feature Metadata

label_name: ndhC-tRNA-Val(UAC)
feature_type: igs
region: LSC
length_bp: 1104
variable_sites: 143
pi: 0.141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-tRNA-Val(UAC)_p1	TCATATTCGTGAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	36.4	50.0	56.1	57.8	1125-1160 bp	1.0	59.3
ndhC-tRNA-Val(UAC)_p2	TCATATTCGTGAAGCAGAAACA	GAAGGTCTACGGTTCGAGTC	36.4	55.0	56.1	57.5	1124-1159 bp	1.0	56.6
ndhC-tRNA-Val(UAC)_p3	TCATATTCGTGAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	36.4	55.0	56.1	58.7	1113-1148 bp	1.0	55.4
ndhC-tRNA-Val(UAC)_p4	CATATTCGTGAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	38.1	50.0	54.6	57.8	1124-1159 bp	1.0	55.3
ndhC-tRNA-Val(UAC)_p5	TCATATTCGTGAAGCAGAAACAT	AGAAGGTCTACGGTTCGAGT	34.8	50.0	56.6	57.8	1125-1160 bp	1.0	55.3