Hotspot

tRNA-Phe(GAA)-ndhJ

LSC · 48275-48906 · 632 bp

Back to Trithuria

Pi 0.0918

MarkerSeek score 79.70

Primer available yes

Label tRNA-Phe(GAA)-ndhJ

Feature Metadata

label_name: tRNA-Phe(GAA)-ndhJ
feature_type: igs
region: LSC
length_bp: 632
variable_sites: 57
pi: 0.0918
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Phe(GAA)-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	CGTTCCCAATGTGCCTATGA	55.0	50.0	58.0	58.0	1026-1099 bp	1.0	85.6
tRNA-Phe(GAA)-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	ATCTACGTTCCCAATGTGCC	55.0	50.0	58.0	58.0	1031-1104 bp	1.0	85.6
tRNA-Phe(GAA)-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	AAGATGCAGGGTCGTTCATC	55.0	50.0	58.0	58.0	1182-1255 bp	1.0	85.1
tRNA-Phe(GAA)-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	GAAGATGCAGGGTCGTTCAT	55.0	50.0	58.0	58.0	1183-1256 bp	1.0	85.1
tRNA-Phe(GAA)-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	CCCACCGGCATCTATTTCAA	55.0	50.0	58.0	57.9	1357-1429 bp	1.0	84.4