Hotspot

tRNA-Thr(UGU)-tRNA-Leu(UAA)

LSC · 46892-47320 · 429 bp

Back to Trithuria

Pi 0.0909

MarkerSeek score 81.60

Primer available yes

Label tRNA-Thr(UGU)-tRNA-Leu(UAA)

Feature Metadata

label_name: tRNA-Thr(UGU)-tRNA-Leu(UAA)
feature_type: igs
region: LSC
length_bp: 429
variable_sites: 38
pi: 0.0909
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Thr(UGU)-tRNA-Leu(UAA)_p1	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	50.0	50.0	57.8	58.0	511-550 bp	1.0	85.6
tRNA-Thr(UGU)-tRNA-Leu(UAA)_p2	AAATGCGATGCTCTAACCCC	ACCAATTTCGCCATATCCCC	50.0	50.0	58.3	58.0	483-522 bp	1.0	85.4
tRNA-Thr(UGU)-tRNA-Leu(UAA)_p3	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	519-558 bp	1.0	84.9
tRNA-Thr(UGU)-tRNA-Leu(UAA)_p4	GGTACACAAAAGCCCGCTAT	ACCAATTTCGCCATATCCCC	50.0	50.0	58.3	58.0	532 bp	0.5	57.9
tRNA-Thr(UGU)-tRNA-Leu(UAA)_p5	GGTACACAAAAGCCCGCTAT	TAGCGTCTACCAATTTCGCC	50.0	50.0	58.3	58.1	540 bp	0.5	57.4