Hotspot

tRNA-Thr(GGU)-psbD

LSC · 32077-33475 · 1399 bp

Back to Trithuria

Pi 0.0662

MarkerSeek score 81.20

Primer available yes

Label tRNA-Thr(GGU)-psbD

Feature Metadata

label_name: tRNA-Thr(GGU)-psbD
feature_type: igs
region: LSC
length_bp: 1399
variable_sites: 90
pi: 0.0662
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Thr(GGU)-psbD_p1	GGCGTAAGTCATCGGTTCAA	CAAAAACGAAACGGTCCCTG	50.0	50.0	58.3	57.9	1525-1530 bp	1.0	83.6
tRNA-Thr(GGU)-psbD_p2	GGCGTAAGTCATCGGTTCAA	CACAAAAACGAAACGGTCCC	50.0	50.0	58.3	58.2	1527-1532 bp	1.0	83.1
tRNA-Thr(GGU)-psbD_p3	GGCGTAAGTCATCGGTTCAA	CCACAAAAACGAAACGGTCC	50.0	50.0	58.3	58.2	1528-1533 bp	1.0	83.1
tRNA-Thr(GGU)-psbD_p4	GGCGTAAGTCATCGGTTCAA	CCCACAAAAACGAAACGGTC	50.0	50.0	58.3	58.2	1529-1534 bp	1.0	83.1
tRNA-Thr(GGU)-psbD_p5	GGCGTAAGTCATCGGTTCAA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.3	58.2	1526-1531 bp	1.0	82.5