Hotspot

rps16-tRNA-Gln(UUG)

LSC · 6161-7294 · 1134 bp

Back to Trithuria

Pi 0.0927

MarkerSeek score 85.70

Primer available yes

Label rps16-tRNA-Gln(UUG)

Feature Metadata

label_name: rps16-tRNA-Gln(UUG)
feature_type: igs
region: LSC
length_bp: 1134
variable_sites: 97
pi: 0.0927
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 85.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-tRNA-Gln(UUG)_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1207-1264 bp	1.0	88.6
rps16-tRNA-Gln(UUG)_p2	TCTCATTCAAGTCGCACGTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.9	58.1	1215-1272 bp	1.0	88.4
rps16-tRNA-Gln(UUG)_p3	TGCTTTCTACCGCATCGTTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.2	58.1	1196-1253 bp	1.0	88.2
rps16-tRNA-Gln(UUG)_p4	TTGCTTTCTACCGCATCGTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.2	58.1	1197-1254 bp	1.0	88.1
rps16-tRNA-Gln(UUG)_p5	CTCATTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	1214-1271 bp	1.0	87.9