Hotspot

trnA-UGC-trnI-GAU

SSC · 138260-138323 · 64 bp

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Pi 0.0156

MarkerSeek score 61.50

Primer available yes

Label trnA-UGC-trnI-GAU

Feature Metadata

label_name: trnA-UGC-trnI-GAU
feature_type: igs
region: SSC
length_bp: 64
variable_sites: 1
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnA-UGC-trnI-GAU_p1	CAACGACCCAATTGCAAGAG	TGATTTACTTCACGGGCGAG	50.0	50.0	57.7	58.0	161 bp	1.0	77.8
trnA-UGC-trnI-GAU_p2	CCGCCTGGACAATTAGACAT	TGATTTACTTCACGGGCGAG	50.0	50.0	57.7	58.0	195 bp	1.0	77.7
trnA-UGC-trnI-GAU_p3	CCGCCTGGACAATTAGACAT	ATTGGTTGGATCGTAGGTGC	50.0	50.0	57.7	58.0	217 bp	1.0	77.4
trnA-UGC-trnI-GAU_p4	CAACGACCCAATTGCAAGAG	ATTGGTTGGATCGTAGGTGC	50.0	50.0	57.7	58.0	183 bp	1.0	77.4
trnA-UGC-trnI-GAU_p5	CCGCCTGGACAATTAGACAT	AGGTCTCTGGTTCAAGTCCA	50.0	50.0	57.7	57.9	177 bp	1.0	77.0