Hotspot

rps15-ycf1

SSC · 126517-126898 · 382 bp

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Pi 0.0105

MarkerSeek score 45.50

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 382
variable_sites: 4
pi: 0.0105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	GGCTCAGCTCCAGCATAATT	ACGGCAATATGCTTGTGGAT	50.0	45.0	58.0	57.9	1447-1448 bp	1.0	79.7
rps15-ycf1_p2	AACACCATGCATTGATGGGT	ACGGCAATATGCTTGTGGAT	45.0	45.0	58.1	57.9	1194-1195 bp	1.0	79.5
rps15-ycf1_p3	GAAGCTATACGGCTCAGCTC	ACGGCAATATGCTTGTGGAT	55.0	45.0	57.9	57.9	1457-1458 bp	1.0	79.4
rps15-ycf1_p4	GAGAAGCTATACGGCTCAGC	ACGGCAATATGCTTGTGGAT	55.0	45.0	57.9	57.9	1459-1460 bp	1.0	79.4
rps15-ycf1_p5	GGCTCAGCTCCAGCATAATT	AGACACGGCAATATGCTTGT	50.0	45.0	58.0	57.9	1451-1452 bp	1.0	79.2