Hotspot

rpoA-rps11

LSC · 81348-81426 · 79 bp

Back to Trithrinax

Pi 0.0127

MarkerSeek score 59.80

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 79
variable_sites: 1
pi: 0.0127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	GGGACAGAATGAAACGACCA	AATGGGTGTCGACCTCCTAA	50.0	50.0	57.8	58.1	221 bp	1.0	78.5
rpoA-rps11_p2	GGGACAGAATGAAACGACCA	ATGGGTGTCGACCTCCTAAA	50.0	50.0	57.8	58.1	220 bp	1.0	78.5
rpoA-rps11_p3	GGGACAGAATGAAACGACCA	AGAGATGCAGCATTACGAGC	50.0	50.0	57.8	58.1	299 bp	1.0	77.7
rpoA-rps11_p4	GGGACAGAATGAAACGACCA	ATAATGGGTGTCGACCTCCT	50.0	50.0	57.8	57.8	223 bp	1.0	77.5
rpoA-rps11_p5	CCTTTCATAAGCGGGGACAG	ATGGGTGTCGACCTCCTAAA	55.0	50.0	58.3	58.1	233 bp	1.0	77.3