Hotspot

accD-psaI

LSC · 61275-62046 · 772 bp

Back to Trithrinax

Pi 0.0013
MarkerSeek score 46.90
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
772
variable_sites
1
pi
0.0013
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 CCACGGTTTCTTTCCCTTGA GAGGGTAAGTTGAGATCTGTCA 50.0 45.5 58.0 56.9 835 bp 1.0 55.3
accD-psaI_p2 TCCACGGTTTCTTTCCCTTG GAGGGTAAGTTGAGATCTGTCA 50.0 45.5 58.0 56.9 836 bp 1.0 55.3
accD-psaI_p3 TCAGCTCCACGGTTTCTTTC GAGGGTAAGTTGAGATCTGTCA 50.0 45.5 58.1 56.9 841 bp 1.0 54.5
accD-psaI_p4 CCACGGTTTCTTTCCCTTGA AGGGTAAGTTGAGATCTGTCA 50.0 42.9 58.0 55.7 834 bp 1.0 53.6
accD-psaI_p5 TCCACGGTTTCTTTCCCTTG AGGGTAAGTTGAGATCTGTCA 50.0 42.9 58.0 55.7 835 bp 1.0 53.6