Hotspot

trnS-UGA-psbZ

LSC · 36615-36966 · 352 bp

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Pi 0.00568

MarkerSeek score 52.80

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 352
variable_sites: 2
pi: 0.00568
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAAGACCGGAGCTATCAACC	ACCAACCATCAGAAGAAGCA	55.0	45.0	58.1	57.0	488 bp	1.0	72.2
trnS-UGA-psbZ_p2	CTATCAACCACTCGGCCATC	ACCAACCATCAGAAGAAGCA	55.0	45.0	58.1	57.0	477 bp	1.0	71.6
trnS-UGA-psbZ_p3	CAAGACCGGAGCTATCAACC	GACCAACCATCAGAAGAAGCA	55.0	47.6	58.1	58.2	489 bp	1.0	70.1
trnS-UGA-psbZ_p4	CAAGACCGGAGCTATCAACC	TGACCAACCATCAGAAGAAGC	55.0	47.6	58.1	58.2	490 bp	1.0	70.1
trnS-UGA-psbZ_p5	CAAGACCGGAGCTATCAACC	ACCAACCATCAGAAGAAGCAA	55.0	42.9	58.1	57.8	488 bp	1.0	70.0