Hotspot

trnS-GCU-trnG-GCC

LSC · 8382-9654 · 1273 bp

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Pi 0.00157

MarkerSeek score 59.70

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 1273
variable_sites: 2
pi: 0.00157
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	1346-1347 bp	1.0	51.6
trnS-GCU-trnG-GCC_p2	GGATTCGAACCCTCGGTATG	ACGAATCACACTTTTACCACT	55.0	38.1	57.8	55.5	1383-1384 bp	1.0	50.7
trnS-GCU-trnG-GCC_p3	ATTAGCAATCCGCCGCTTTA	AGAACGAATCACACTTTTACCAC	45.0	39.1	58.0	57.3	1349-1350 bp	1.0	50.5
trnS-GCU-trnG-GCC_p4	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	45.0	39.1	58.0	57.3	1348-1349 bp	1.0	50.5
trnS-GCU-trnG-GCC_p5	AGAAGAAGCGGAGAGAGAGG	ACGAATCACACTTTTACCACT	55.0	38.1	58.2	55.5	1402-1403 bp	1.0	50.0