Hotspot

rps15-trnN-GUU

IRa · 116742-118322 · 1581 bp

Back to Tristachya

Pi 0.00569

MarkerSeek score 61.90

Primer available yes

Label rps15-trnN-GUU

Feature Metadata

label_name: rps15-trnN-GUU
feature_type: igs
region: IRa
length_bp: 1581
variable_sites: 9
pi: 0.00569
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-trnN-GUU_p1	AATGTGAAGCAAGTCTCCGT	ACGACCAGTCAGTTAACAGC	45.0	50.0	57.4	57.8	1721 bp	1.0	74.8
rps15-trnN-GUU_p2	AATGTGAAGCAAGTCTCCGT	AGGATTCGAACCTACGACCA	45.0	50.0	57.4	58.2	1734 bp	1.0	74.8
rps15-trnN-GUU_p3	AATGTGAAGCAAGTCTCCGT	GGATTCGAACCTACGACCAG	45.0	55.0	57.4	57.8	1733 bp	1.0	74.4
rps15-trnN-GUU_p4	AATGTGAAGCAAGTCTCCGT	CCCCAAGTAGGATTCGAACC	45.0	55.0	57.4	57.7	1742 bp	1.0	73.4
rps15-trnN-GUU_p5	AATGTGAAGCAAGTCTCCGT	TTCGAACCTACGACCAGTCA	45.0	50.0	57.4	58.4	1730 bp	1.0	72.9