Hotspot

rpl32-trnL-UAG

SSC · 106962-107486 · 525 bp

Back to Tristachya

Pi 0.0751

MarkerSeek score 81.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 525
variable_sites: 39
pi: 0.0751
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CAACGAGCATCCAAAACCAA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.2	57.9	611-613 bp	1.0	78.6
rpl32-trnL-UAG_p2	CAACGAGCATCCAAAACCAA	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.2	57.8	604-606 bp	1.0	78.1
rpl32-trnL-UAG_p3	CAACGAGCATCCAAAACCAA	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	57.2	57.8	610-612 bp	1.0	78.0
rpl32-trnL-UAG_p4	CAACGAGCATCCAAAACCAA	CTAAGAGCAGCGTGTCTACC	45.0	55.0	57.2	57.8	609-611 bp	1.0	78.0
rpl32-trnL-UAG_p5	CAACGAGCATCCAAAACCAA	GCTTGAGCACTGCTTCCTAA	45.0	50.0	57.2	58.2	625-627 bp	1.0	77.9