Hotspot

trnN-GUU-rps15

IRb · 101803-103383 · 1581 bp

Back to Tristachya

Pi 0.00569
MarkerSeek score 61.90
Primer available yes
Label trnN-GUU-rps15

Feature Metadata

label_name
trnN-GUU-rps15
feature_type
igs
region
IRb
length_bp
1581
variable_sites
9
pi
0.00569
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-rps15_p1 ACGACCAGTCAGTTAACAGC AATGTGAAGCAAGTCTCCGT 50.0 45.0 57.8 57.4 1721 bp 1.0 74.8
trnN-GUU-rps15_p2 AGGATTCGAACCTACGACCA AATGTGAAGCAAGTCTCCGT 50.0 45.0 58.2 57.4 1734 bp 1.0 74.8
trnN-GUU-rps15_p3 GGATTCGAACCTACGACCAG AATGTGAAGCAAGTCTCCGT 55.0 45.0 57.8 57.4 1733 bp 1.0 74.4
trnN-GUU-rps15_p4 CCCCAAGTAGGATTCGAACC AATGTGAAGCAAGTCTCCGT 55.0 45.0 57.7 57.4 1742 bp 1.0 73.4
trnN-GUU-rps15_p5 TTCGAACCTACGACCAGTCA AATGTGAAGCAAGTCTCCGT 50.0 45.0 58.4 57.4 1730 bp 1.0 72.9