Hotspot

ycf3-trnS-GGA

LSC · 44614-45200 · 587 bp

Back to Tristachya

Pi 0.019

MarkerSeek score 63.70

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 587
variable_sites: 11
pi: 0.019
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTGGCTACAATCGAGGAGGT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.4	58.2	725-734 bp	1.0	77.5
ycf3-trnS-GGA_p2	TTGGCTACAATCGAGGAGGT	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.4	57.8	675-684 bp	1.0	77.4
ycf3-trnS-GGA_p3	GGCTACAATCGAGGAGGTCT	ACGGAAAGAGAGGGATTCGA	55.0	50.0	58.6	58.2	723-732 bp	1.0	76.2
ycf3-trnS-GGA_p4	GGCTACAATCGAGGAGGTCT	TTCCAATGCTACGCCTTGAA	55.0	45.0	58.6	57.8	673-682 bp	1.0	76.1
ycf3-trnS-GGA_p5	TTGGCTACAATCGAGGAGGT	AGTTCCAATGCTACGCCTTG	50.0	50.0	58.4	58.5	677-686 bp	1.0	74.5