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psbM-petN

LSC · 16897-17684 · 788 bp

Back to Tristachya

Pi 0.0194

MarkerSeek score 60.10

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 788
variable_sites: 13
pi: 0.0194
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	AAAACAGCCAGCCAAAATGA	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.7	58.2	779-895 bp	1.0	73.6
psbM-petN_p2	AAAACAGCCAGCCAAAATGA	GTAGTATGGGGGAGGAGTGG	40.0	60.0	56.7	58.3	723-839 bp	1.0	72.7
psbM-petN_p3	AAAACAGCCAGCCAAAATGA	TAGTATGGGGGAGGAGTGGA	40.0	55.0	56.7	58.4	722-838 bp	1.0	72.0
psbM-petN_p4	AAACAGCCAGCCAAAATGAT	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.5	58.2	778-894 bp	1.0	71.8
psbM-petN_p5	AACAGCCAGCCAAAATGATT	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.5	58.2	777-893 bp	1.0	71.8