Hotspot

trnG-UCC-trnM-CAU

LSC · 13771-14620 · 850 bp

Back to Tristachya

Pi 0.025

MarkerSeek score 62.80

Primer available yes

Label trnG-UCC-trnM-CAU

Feature Metadata

label_name: trnG-UCC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 850
variable_sites: 21
pi: 0.025
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnM-CAU_p1	ACGAATCACACTTTTACCACT	ATTCGAACCGACGACTTCTG	38.1	50.0	55.5	58.0	993-1004 bp	1.0	54.1
trnG-UCC-trnM-CAU_p2	AGAACGAATCACACTTTTACCAC	ATTCGAACCGACGACTTCTG	39.1	50.0	57.3	58.0	996-1007 bp	1.0	53.0
trnG-UCC-trnM-CAU_p3	GAACGAATCACACTTTTACCACT	ATTCGAACCGACGACTTCTG	39.1	50.0	57.3	58.0	995-1006 bp	1.0	53.0
trnG-UCC-trnM-CAU_p4	ACGAATCACACTTTTACCACT	CCGACGACTTCTGTCTTACC	38.1	55.0	55.5	57.7	986-997 bp	1.0	52.2
trnG-UCC-trnM-CAU_p5	AACGAATCACACTTTTACCACT	ATTCGAACCGACGACTTCTG	36.4	50.0	56.2	58.0	994-1005 bp	1.0	52.2