Hotspot

rpl16

LSC · 77463-78914 · 1452 bp

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Pi 0.011
MarkerSeek score 64.10
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1452
variable_sites
24
pi
0.011
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 CAGAAAGAAAAACAGGGCGT TCCTGAAATCCCTGGAAAAA 45.0 40.0 56.8 54.4 1596-1600 bp 0.666667 29.1
rpl16_p2 CAGAAAGAAAAACAGGGCGT TTCCTGAAATCCCTGGAAAA 45.0 40.0 56.8 54.4 1597-1601 bp 0.666667 29.1
rpl16_p3 CAGAAAGAAAAACAGGGCGT TTTCCTGAAATCCCTGGAAA 45.0 40.0 56.8 54.4 1598-1602 bp 0.666667 29.1
rpl16_p4 CAGAAAGAAAAACAGGGCGT TTTTCCTGAAATCCCTGGAA 45.0 40.0 56.8 54.4 1599-1603 bp 0.666667 29.1
rpl16_p5 CAGAAAGAAAAACAGGGCGT TTTTTCCTGAAATCCCTGGA 45.0 40.0 56.8 54.4 1600-1604 bp 0.666667 29.1