Hotspot

rpl32-trnL-UAG

SSC · 105274-105904 · 631 bp

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Pi 0.0264
MarkerSeek score 62.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
631
variable_sites
25
pi
0.0264
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGCAACAACAACAAACAAA TTCGAACCGAGATGCTTGAG 40.0 50.0 56.1 58.0 727-737 bp 1.0 67.2
rpl32-trnL-UAG_p2 GGGCAACAACAACAAACAAA GGATTCGAACCGAGATGCTT 40.0 50.0 56.1 58.1 730-740 bp 1.0 66.8
rpl32-trnL-UAG_p3 GGGCAACAACAACAAACAAA TCCTAAGAGCAGCGTGTCTA 40.0 50.0 56.1 57.9 700-710 bp 1.0 66.4
rpl32-trnL-UAG_p4 GGGCAACAACAACAAACAAA AGCAGCGTGTCTACCAATTT 40.0 45.0 56.1 57.8 693-703 bp 1.0 65.9
rpl32-trnL-UAG_p5 GGGCAACAACAACAAACAAA GCTTGAGCACTGCTTCCTAA 40.0 50.0 56.1 58.2 714-724 bp 1.0 65.8