Hotspot

rpl22-rps19

IRb · 80291-80365 · 75 bp

Back to Trisetum

Pi 0.0311

MarkerSeek score 63.70

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: IRb
length_bp: 75
variable_sites: 3
pi: 0.0311
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	ACTTTACTAGTTTGAAACTTGTCA	CAAATTGGGGGAATTCGTGC	29.2	50.0	54.6	58.0	164-182 bp	1.0	45.4
rpl22-rps19_p2	ACTTTACTAGTTTGAAACTTGTCA	GCAAATTGGGGGAATTCGTG	29.2	50.0	54.6	58.0	165-183 bp	1.0	45.4
rpl22-rps19_p3	ACTTTACTAGTTTGAAACTTGTCAT	CAAATTGGGGGAATTCGTGC	28.0	50.0	55.1	58.0	164-182 bp	1.0	45.4
rpl22-rps19_p4	ACTTTACTAGTTTGAAACTTGTCA	ATGGTAGGTCGCAAATTGGG	29.2	50.0	54.6	58.2	175-193 bp	1.0	45.3
rpl22-rps19_p5	ACTTTACTAGTTTGAAACTTGTCA	GTCGCAAATTGGGGGAATTC	29.2	50.0	54.6	57.7	168-186 bp	1.0	45.3