Hotspot

rps11-rpl36

LSC · 75607-75785 · 179 bp

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Pi 0.0149

MarkerSeek score 61.50

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 179
variable_sites: 4
pi: 0.0149
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CGTGCACTTTTCCGTAGACT	GCGTGGACGAATTAGAGTCA	50.0	50.0	58.2	57.7	329-336 bp	1.0	77.0
rps11-rpl36_p2	TTCTACGTGCACTTTTCCGT	GCGTGGACGAATTAGAGTCA	45.0	50.0	57.8	57.7	334-341 bp	1.0	76.9
rps11-rpl36_p3	CGTGCACTTTTCCGTAGACT	GGCGTGGACGAATTAGAGTC	50.0	55.0	58.2	58.4	330-337 bp	1.0	75.7
rps11-rpl36_p4	TTCTACGTGCACTTTTCCGT	GGCGTGGACGAATTAGAGTC	45.0	55.0	57.8	58.4	335-342 bp	1.0	75.6
rps11-rpl36_p5	CGTGCACTTTTCCGTAGACT	CGTAGGCGTGGACGAATTAG	50.0	55.0	58.2	58.5	334-341 bp	1.0	75.0