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clpP1-psbB

LSC · 68078-68611 · 534 bp

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Pi 0.00749

MarkerSeek score 62.20

Primer available yes

Label clpP1-psbB

Feature Metadata

label_name: clpP1-psbB
feature_type: igs
region: LSC
length_bp: 534
variable_sites: 6
pi: 0.00749
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1-psbB_p1	ACCCAAGTCGCTTCTTCATC	TATAAAGCCATCGAGCCAGC	50.0	50.0	57.9	57.8	715 bp	1.0	78.5
clpP1-psbB_p2	TCAACCCAAGTCGCTTCTTC	TATAAAGCCATCGAGCCAGC	50.0	50.0	58.1	57.8	718 bp	1.0	78.3
clpP1-psbB_p3	ACCCAAGTCGCTTCTTCATC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.9	58.2	616 bp	1.0	78.1
clpP1-psbB_p4	CTTTTGGAACACCAATGGGC	TATAAAGCCATCGAGCCAGC	50.0	50.0	57.8	57.8	675 bp	1.0	78.0
clpP1-psbB_p5	TCAACCCAAGTCGCTTCTTC	CGATACCAAGGCAAACCCAT	50.0	50.0	58.1	58.2	619 bp	1.0	77.9