Hotspot

psbE-petL

LSC · 62018-63270 · 1253 bp

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Pi 0.0106

MarkerSeek score 64.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1253
variable_sites: 20
pi: 0.0106
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	GCCAATAAATAGAGCTGGGGT	45.0	47.6	58.0	57.8	1439-1463 bp	1.0	71.2
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GCCAATAAATAGAGCTGGGGT	50.0	47.6	57.9	57.8	1428-1452 bp	1.0	71.0
psbE-petL_p3	ACCAACCCGCAATGAATAGG	GCCAATAAATAGAGCTGGGGT	50.0	47.6	58.2	57.8	1430-1454 bp	1.0	69.5
psbE-petL_p4	TGACAAATAACCAACCCGCA	AGCCAATAAATAGAGCTGGGG	45.0	47.6	58.0	57.5	1440-1464 bp	1.0	69.0
psbE-petL_p5	CAACCCGCAATGAATAGGGA	AGCCAATAAATAGAGCTGGGG	50.0	47.6	57.9	57.5	1429-1453 bp	1.0	68.7