Hotspot

petA-psbJ

LSC · 60443-61248 · 806 bp

Back to Trisetum

Pi 0.0186

MarkerSeek score 71.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 806
variable_sites: 22
pi: 0.0186
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	TCCTCTTTGGCTGATAGGTACT	50.0	45.5	57.9	58.0	1003-1010 bp	1.0	65.9
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	CCTCTTTGGCTGATAGGTACTG	50.0	50.0	57.9	57.9	1002-1009 bp	1.0	65.4
petA-psbJ_p3	TGTTCTTCTTCGCATCCGTT	TCCTCTTTGGCTGATAGGTACT	45.0	45.5	57.8	58.0	996-1003 bp	1.0	65.0
petA-psbJ_p4	TTGTTCTTCTTCGCATCCGT	TCCTCTTTGGCTGATAGGTACT	45.0	45.5	57.8	58.0	997-1004 bp	1.0	65.0
petA-psbJ_p5	TGTTCTTCTTCGCATCCGTT	CCTCTTTGGCTGATAGGTACTG	45.0	50.0	57.8	57.9	995-1002 bp	1.0	64.5