Hotspot

rbcL-rpl23

LSC · 55623-55877 · 255 bp

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Pi 0.0183

MarkerSeek score 50.10

Primer available yes

Label rbcL-rpl23

Feature Metadata

label_name: rbcL-rpl23
feature_type: igs
region: LSC
length_bp: 255
variable_sites: 7
pi: 0.0183
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-rpl23_p1	GATCATATCCACGCCGGTAC	CTGTTAGTAAGACCCCGTGC	55.0	55.0	58.0	58.0	1409-1437 bp	1.0	82.2
rbcL-rpl23_p2	ATATCCACGCCGGTACAGTA	CTGTTAGTAAGACCCCGTGC	50.0	55.0	58.0	58.0	1405-1433 bp	1.0	82.2
rbcL-rpl23_p3	TCTTCACATTCACCGTGCAA	CTGTTAGTAAGACCCCGTGC	45.0	55.0	58.0	58.0	1509-1537 bp	1.0	81.8
rbcL-rpl23_p4	TTCTTCACATTCACCGTGCA	CTGTTAGTAAGACCCCGTGC	45.0	55.0	58.0	58.0	1510-1538 bp	1.0	81.8
rbcL-rpl23_p5	GCATGACTACATCACTGGGG	CTGTTAGTAAGACCCCGTGC	55.0	55.0	58.0	58.0	1581-1609 bp	1.0	81.7