Hotspot

petB

LSC · 78250-79651 · 1402 bp

Back to Tripterygium

Pi 0.00571

MarkerSeek score 51.50

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1402
variable_sites: 8
pi: 0.00571
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	AGTTCGACCGCGGAATTTTT	TCTTCTCCCCCAAGTGATCA	45.0	50.0	58.8	57.7	1501-1517 bp	1.0	71.5
petB_p2	AGTTCGACCGCGGAATTTTT	ACTCTTCTCCCCCAAGTGAT	45.0	50.0	58.8	57.6	1503-1519 bp	1.0	71.5
petB_p3	AGTTCGACCGCGGAATTTT	TCTTCTCCCCCAAGTGATCA	47.4	50.0	58.1	57.7	1501-1517 bp	1.0	69.1
petB_p4	AGTTCGACCGCGGAATTTT	ACTCTTCTCCCCCAAGTGAT	47.4	50.0	58.1	57.6	1503-1519 bp	1.0	69.0
petB_p5	TTTTGGGTCCGTTTTGGCA	TCTTCTCCCCCAAGTGATCA	47.4	50.0	58.4	57.7	1519-1535 bp	1.0	66.4