Hotspot

cemA

LSC · 64273-64962 · 690 bp

Back to Tripterygium

Pi 0.0058

MarkerSeek score 62.10

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 690
variable_sites: 4
pi: 0.0058
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	GGGATGCCTTGATACCTTGG	GAATTGGGGCAGTGGATCAT	55.0	50.0	58.0	57.9	818 bp	1.0	79.7
cemA_p2	GGGGATGCCTTGATACCTTG	GAATTGGGGCAGTGGATCAT	55.0	50.0	58.0	57.9	819 bp	1.0	79.7
cemA_p3	GGGATGCCTTGATACCTTGG	ACGTTAGAATTGGGGCAGTG	55.0	50.0	58.0	58.2	824 bp	1.0	78.9
cemA_p4	GGGGATGCCTTGATACCTTG	ACGTTAGAATTGGGGCAGTG	55.0	50.0	58.0	58.2	825 bp	1.0	78.9
cemA_p5	GGGATGCCTTGATACCTTGG	TTGGGGCAGTGGATCATTTT	55.0	45.0	58.0	57.7	815 bp	1.0	77.7