Hotspot

ndhF-rpl32

SSC · 116409-117343 · 935 bp

Back to Tripterygium

Pi 0.00432

MarkerSeek score 60.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 935
variable_sites: 4
pi: 0.00432
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCCCCACTATTCCCATAGGA	TTTCAACGCTGCCCAAAATC	55.0	45.0	57.6	57.9	1098-1099 bp	1.0	76.3
ndhF-rpl32_p2	CCCCCACTATTCCCATAGGA	CTTTCAACGCTGCCCAAAAT	55.0	45.0	57.6	57.8	1099-1100 bp	1.0	76.3
ndhF-rpl32_p3	CCCCCACTATTCCCATAGGA	CGTAGCAAGGGATTTCGCTA	55.0	50.0	57.6	57.8	1128-1129 bp	1.0	75.7
ndhF-rpl32_p4	CCCCCACTATTCCCATAGGA	TGAAAAGGCTTTCAACGCTG	55.0	45.0	57.6	57.5	1107-1108 bp	1.0	73.5
ndhF-rpl32_p5	CCCCCACTATTCCCATAGGA	GCTGCCCAAAATCCCTTTCT	55.0	50.0	57.6	58.7	1091-1092 bp	1.0	71.6