Hotspot

infA-rps8

LSC · 83413-83559 · 147 bp

Back to Tripterygium

Pi 0.0204

MarkerSeek score 64.20

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 147
variable_sites: 3
pi: 0.0204
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	AACGAACCCGAAACATACCA	TCGGCGGAGAAATTTTGTGT	45.0	45.0	57.5	58.4	252-254 bp	1.0	73.4
infA-rps8_p2	AACGAACCCGAAACATACCA	CGGCGGAGAAATTTTGTGTT	45.0	45.0	57.5	57.6	251-253 bp	1.0	73.2
infA-rps8_p3	AACGAACCCGAAACATACCA	GCATCGGCGGAGAAATTTTG	45.0	50.0	57.5	58.5	255-257 bp	1.0	73.0
infA-rps8_p4	ACGAACCCGAAACATACCAT	TCGGCGGAGAAATTTTGTGT	45.0	45.0	57.2	58.4	251-253 bp	1.0	71.6
infA-rps8_p5	ACGAACCCGAAACATACCAT	CGGCGGAGAAATTTTGTGTT	45.0	45.0	57.2	57.6	250-252 bp	1.0	71.4