Hotspot

psbZ-trnG-GCC

LSC · 36571-37268 · 698 bp

Back to Tripterygium

Pi 0.0143

MarkerSeek score 67.10

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 698
variable_sites: 10
pi: 0.0143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.7	57.8	856-870 bp	1.0	77.4
psbZ-trnG-GCC_p2	TGAGGGTTGGTCGAGTAACA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.3	57.8	845-859 bp	1.0	77.3
psbZ-trnG-GCC_p3	CTGAGGGTTGGTCGAGTAAC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	57.6	57.8	846-860 bp	1.0	76.7
psbZ-trnG-GCC_p4	CCTGAGGGTTGGTCGAGTAA	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.7	57.8	847-861 bp	1.0	73.8
psbZ-trnG-GCC_p5	GAGGGTTGGTCGAGTAACAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.2	57.8	844-858 bp	1.0	73.1