Hotspot

trnE-UUC-trnT-GGU

LSC · 31116-31726 · 611 bp

Back to Tripterygium

Pi 0.0458

MarkerSeek score 85.10

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 611
variable_sites: 28
pi: 0.0458
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	714 bp	1.0	81.0
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	693 bp	1.0	80.9
trnE-UUC-trnT-GGU_p3	TGGGCTTACTGAGAATCCCT	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	785 bp	1.0	80.6
trnE-UUC-trnT-GGU_p4	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.3	58.1	693 bp	1.0	80.6
trnE-UUC-trnT-GGU_p5	TGGGCTTACTGAGAATCCCT	CCATGGCGTTACTCTACCAC	50.0	55.0	57.7	58.1	764 bp	1.0	80.3