Hotspot

psbM-trnD-GUC

LSC · 29921-30384 · 464 bp

Back to Tripterygium

Pi 0.00649

MarkerSeek score 60.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 464
variable_sites: 3
pi: 0.00649
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	CGCCCTGACTTTGACTGATA	TTCAATTGGTCAGAGCACCG	50.0	50.0	57.3	58.5	632-634 bp	1.0	71.4
psbM-trnD-GUC_p2	TATCGCCCTGACTTTGACTG	TTCAATTGGTCAGAGCACCG	50.0	50.0	57.3	58.5	635-637 bp	1.0	71.4
psbM-trnD-GUC_p3	ATCGCCCTGACTTTGACTGA	TTCAATTGGTCAGAGCACCG	50.0	50.0	59.0	58.5	634-636 bp	1.0	68.6
psbM-trnD-GUC_p4	TCGCCCTGACTTTGACTGAT	TTCAATTGGTCAGAGCACCG	50.0	50.0	59.0	58.5	633-635 bp	1.0	68.6
psbM-trnD-GUC_p5	TTATCGCCCTGACTTTGACTG	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.0	58.5	636-638 bp	1.0	68.6