Hotspot

rpoA

LSC · 76924-77934 · 1011 bp

Back to Tripterospermum

Pi 0.00297

MarkerSeek score 53.50

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1011
variable_sites: 7
pi: 0.00297
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	GCCCTAGATACGCCTAGTCA	GGGGCTGTCAAGAGAAACAA	55.0	50.0	58.1	58.0	1179-1180 bp	1.0	79.6
rpoA_p2	GCCCTAGATACGCCTAGTCA	AAATGTACGCTAAGGGGCTG	55.0	50.0	58.1	58.0	1192-1193 bp	1.0	79.5
rpoA_p3	AGCCCTAGATACGCCTAGTC	GGGGCTGTCAAGAGAAACAA	55.0	50.0	57.8	58.0	1180-1181 bp	1.0	78.8
rpoA_p4	AGCCCTAGATACGCCTAGTC	AAATGTACGCTAAGGGGCTG	55.0	50.0	57.8	58.0	1193-1194 bp	1.0	78.6
rpoA_p5	GCCCTAGATACGCCTAGTCA	AAGGGGCTGTCAAGAGAAAC	55.0	50.0	58.1	57.7	1181-1182 bp	1.0	77.6