Hotspot

rpl16

LSC · 79913-81103 · 1191 bp

Back to Tripterospermum

Pi 0.00118

MarkerSeek score 41.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1191
variable_sites: 3
pi: 0.00118
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 41.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GCGAAGAACCATTTTTGTTGG	AGGGTTGAATAAAAATTTGACTGA	42.9	29.2	56.9	55.2	1260-1270 bp	1.0	40.8
rpl16_p2	AGCGAAGAACCATTTTTGTTGG	AGGGTTGAATAAAAATTTGACTGA	40.9	29.2	58.3	55.2	1261-1271 bp	1.0	40.8
rpl16_p3	GCGAAGAACCATTTTTGTTGG	AGGGTTGAATAAAAATTTGACTGATCT	42.9	29.6	56.9	57.9	1260-1270 bp	1.0	40.8
rpl16_p4	GCGAAGAACCATTTTTGTTGG	TGACTGATCTTTTCATAAAATTGCT	42.9	28.0	56.9	55.8	1243-1253 bp	1.0	40.8
rpl16_p5	GCGAAGAACCATTTTTGTTGG	GGGTTGAATAAAAATTTGACTGA	42.9	30.4	56.9	53.8	1259-1269 bp	1.0	40.8