Hotspot

trnR-ACG-rrn5

IRa · 127658-127918 · 261 bp

Back to Tripterospermum

Pi 0.00153
MarkerSeek score 38.70
Primer available yes
Label trnR-ACG-rrn5

Feature Metadata

label_name
trnR-ACG-rrn5
feature_type
igs
region
IRa
length_bp
261
variable_sites
1
pi
0.00153
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
38.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-rrn5_p1 CTTTTGGTCCGCTATTCCCA GGTGACGATACTGTAGGGGA 50.0 55.0 57.9 57.9 421 bp 1.0 78.7
trnR-ACG-rrn5_p2 CCACGTGCTCTAATCCTCTG GGTGACGATACTGTAGGGGA 55.0 55.0 57.8 57.9 345 bp 1.0 78.0
trnR-ACG-rrn5_p3 CTTTTGGTCCGCTATTCCCA ATTCTGGTGTCCTAGGCGTA 50.0 50.0 57.9 57.8 489 bp 1.0 77.9
trnR-ACG-rrn5_p4 CTTTTGGTCCGCTATTCCCA GTAGAGGAACCACACCAACC 50.0 55.0 57.9 57.8 472 bp 1.0 77.7
trnR-ACG-rrn5_p5 AGCTTTTGGTCCGCTATTCC GGTGACGATACTGTAGGGGA 50.0 55.0 58.2 57.9 423 bp 1.0 77.7