Hotspot

rpl32-trnL-UAG

SSC · 111202-112023 · 822 bp

Back to Tripterospermum

Pi 0.00609
MarkerSeek score 52.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
822
variable_sites
11
pi
0.00609
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGTGCAGCAAACAAAAACAA TCCTAAGAGCAGCGTGTCTA 35.0 50.0 55.7 57.9 847-889 bp 1.0 65.5
rpl32-trnL-UAG_p2 TTGTGCAGCAAACAAAAACA TCCTAAGAGCAGCGTGTCTA 35.0 50.0 55.7 57.9 848-890 bp 1.0 65.5
rpl32-trnL-UAG_p3 TGTGCAGCAAACAAAAACAA AGCAGCGTGTCTACCAATTT 35.0 45.0 55.7 57.8 840-882 bp 1.0 65.0
rpl32-trnL-UAG_p4 TTGTGCAGCAAACAAAAACA AGCAGCGTGTCTACCAATTT 35.0 45.0 55.7 57.8 841-883 bp 1.0 65.0
rpl32-trnL-UAG_p5 TGTGCAGCAAACAAAAACAA CTAAGAGCAGCGTGTCTACC 35.0 55.0 55.7 57.8 845-887 bp 1.0 64.9