Hotspot

psbE-petL

LSC · 63773-64805 · 1033 bp

Back to Tripterospermum

Pi 0.00357

MarkerSeek score 63.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1033
variable_sites: 9
pi: 0.00357
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	ACGAATAACCAACCCGCAAT	TCGTTAAAGCCGCGAGTAAA	45.0	45.0	58.2	57.9	1168-1195 bp	1.0	80.6
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	TCGTTAAAGCCGCGAGTAAA	55.0	45.0	58.2	57.9	1077-1104 bp	1.0	80.6
psbE-petL_p3	TGCTGACGAATAACCAACCC	TCGTTAAAGCCGCGAGTAAA	50.0	45.0	58.2	57.9	1173-1200 bp	1.0	80.5
psbE-petL_p4	CGAATAACCAACCCGCAATG	TCGTTAAAGCCGCGAGTAAA	50.0	45.0	57.8	57.9	1167-1194 bp	1.0	80.4
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	TCGTTAAAGCCGCGAGTAAA	50.0	45.0	58.4	57.9	1075-1102 bp	1.0	79.3