Hotspot

ndhC-trnV-UAC

LSC · 50088-50913 · 826 bp

Back to Tripterospermum

Pi 0.00103
MarkerSeek score 55.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
826
variable_sites
2
pi
0.00103
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.94
markerseek_score
55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCGGTCCTTTCCTAATTGGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 57.9 57.8 897-974 bp 1.0 82.2
ndhC-trnV-UAC_p2 CGGTCCTTTCCTAATTGGGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 57.9 57.8 896-973 bp 1.0 82.2
ndhC-trnV-UAC_p3 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 880-957 bp 1.0 81.5
ndhC-trnV-UAC_p4 AATTGGGGCTAAAACTCCGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.2 57.8 884-961 bp 1.0 81.4
ndhC-trnV-UAC_p5 GGTCCTTTCCTAATTGGGGC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.2 57.8 895-972 bp 1.0 80.8