Pi
0.00378
MarkerSeek score
56.80
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | AGGGGCAGTTTCAATCATGG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 820-830 bp | 1.0 | 80.6 |
| trnT-UGU-trnL-UAA_p2 | GGGGCAGTTTCAATCATGGA | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 819-829 bp | 1.0 | 80.5 |
| trnT-UGU-trnL-UAA_p3 | AGGGGCAGTTTCAATCATGG | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.1 | 58.1 | 828-838 bp | 1.0 | 80.0 |
| trnT-UGU-trnL-UAA_p4 | GGGGCAGTTTCAATCATGGA | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.1 | 58.1 | 827-837 bp | 1.0 | 80.0 |
| trnT-UGU-trnL-UAA_p5 | TAGGAAAAAGCCGGCTATCG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 786-796 bp | 1.0 | 79.9 |