Hotspot

psaA-ycf3

LSC · 41462-42292 · 831 bp

Back to Tripterospermum

Pi 0.00385

MarkerSeek score 51.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 831
variable_sites: 8
pi: 0.00385
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TTTTACTTCTGGTTCCGGCG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.5	58.0	962-972 bp	1.0	78.3
psaA-ycf3_p2	TGTTCTTGAGAAATGGCCCG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.5	58.0	1034-1044 bp	1.0	78.2
psaA-ycf3_p3	TTTTACTTCTGGTTCCGGCG	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.5	57.8	891-901 bp	1.0	77.5
psaA-ycf3_p4	TGTTCTTGAGAAATGGCCCG	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.5	57.8	963-973 bp	1.0	77.4
psaA-ycf3_p5	TGTTCTTGAGAAATGGCCCG	TTGGTTGAAGATCACGAGGC	50.0	50.0	58.5	58.2	967-977 bp	1.0	77.2